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Thermo Scientific Pierce Streptavidin and Conjugates include purified recombinant streptavidin biotin-binding protein, plus standard SA-HRP, SA-AP and fluorescent conjugates for biotin-based assays.

These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates （peroxidase-conjugated streptavidin） are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.

Highlights:

• Recombinant streptavidin – high-quality protein, 53kDa, pI = 6.8 to 7.5
• Pure protein and enzyme conjugates – choose pure, unconjugated protein for biotin blocking and controls; enzyme conjugates for substrate-based detection
• Fluorophore conjugates – standard fluorescent forms （fluorescein, rhodamine, etc.） for traditional immunofluorescence techniques

• Activated preparations – choose a maleimide-activated or hydrazide-activated form to prepare a custom streptavidin conjugate with sulfhydryl- or carbohydrate-containing molecule